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RE: How to use mask on CONN

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[i]Originally posted by Daniel Santos:[/i][quote]Hi,

I work with stroke patients and I dont know in which step should I insert the lesion masks on conn. Someone could help with this information? 

- How can I work with lesion masks on conn and how would be this process?[/quote][color=#000000]Daniel, [/color]

[color=#000000]Did you ever receive a response to your above question? I ask because I have a similar question.[/color]

[color=#000000]-Kate[/color]

Lesion Segmentation

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Hello CONN-ers,

I'm attempting to use CONN for ROI-ROI and seed-voxel analyses. What are people using with regards to lesion segmentation? Are people excluding lesion voxels from the computation...additional regression...if so, where in the processing pipeline is this occurring (i.e. before or after transformation to atlas space)? Any insight would be greatly appreciated.

-KJ

ROI.mat help

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Hi

So i'm trying to do a roi-roi and plot it's connectivity matrix in python. So as i gather the roi.mat is produced when i clicked on result explorer. I've chosen a source however i only want to select about 10-20 targets not all 164 that conn uses. How can i do this easily? So how can i get only 10-20 targets in my roi.mat. Can i do this? Sorry for the newbie question. Thank you for any help.

Kevin

When preprocessing stopps (disc space issue)

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Dear all,

My preprocessing pipeline (1st, standard) stopped in the middle of functional segmentation & normalisation because of lack of space. Segments finished correctly. Since this is quite a big amount of data, how to continue now correctly and avoid double files?

I would appreciate your advise
Greetings,
Lucas

RE: ROI-to-ROI value extraction

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Martin,

Thanks! That worked, and these are the same values that I got through the method that I gave so that gives me reassurance that numbers are what I think they are. I believe that the file that I spoke about is created after you click on results explorer with the relevant second-level contrast designated.

Best,

Greg

Atlas.nii ROI's

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Hello,

Does anyone know if there is any information on the size, shape, and exact locations (MNI coordinates) of the ROI's in atlas.nii file within conn. I have tried to separate the ROIs in this image into different files, but I have been unsuccessful. There is a .mat file within the results/firstlevel/([specific analysis] folder that contains a variable xyz that gives MNI coordinates for each ROI. Plotting these points gives a region that appears to be the center of the ROI. Can anyone confirm this?

Thank you in advance for your help!

Best,

Greg

RE: Set up - MNI Boundaries

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[i]Originally posted by Stephen L.:[/i][quote]Dear Jihyun,

The boundaries overall look fine to me, except as you say on the mid-top slices where the boundaries seem to be missing, which is weird, and I have never seen that.

Can you please try to do the same check on the structural? Also is this on the average of functional connectivity of all subjects or only on one specific subject? If the latter, is the same "bug" happening on other subjects as well?

Hope this helps,
Stephen[/quote][color=#000000]Hi Stephen,[/color]

[color=#000000]Thanks for your reply.[/color]
[color=#000000]The MNI boundaries on structural look same as the one on functional.[/color]
[color=#000000]When I look at the single slice level, the boundaries seem to be present but very sparse and not solid thick yellow line. [/color]

Will this impact the analysis?

Kind Regarsd,

Jihyun

Using other atlas for 2nd level results

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Dear all

I would like to use another atlas for Seed-to-Voxel second-level analysis (other than atlas.nii). Actually I have an own atlas I would like to use, but I don't only have programs using my atlas, but also the "networks.nii" atlas which comes with the toolbox. The error I get is:

"Error using pinv
Too many input arguments.

Error in conn_vproject>vproject_display (line 896)
refsrois.data = spm_get_data(refsrois.V,pinv(refsrois.V.mat)*mat{1}*[xyz,ones(size(xyz,1),1)]')

I have the supposition this is because atlas.nii is a 3D Nifti volume, yielding a 1x1 refsrois.V.mat structure and other atlases such as networks.nii are 4D Nifti, yielding mx1 refsrois.V.mat structures (m being the number of volumes of the Nifti). I tried to work around it by only choosing the first structure (refsrois.V(1).mat), however this led to other errors at a later point in the script.

Do you have an idea how to solve this problem? I would much appreciate it.

Best regards
Tudor

peak voxel Z-value or T-Value

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Hello,

How does one obtain the peak voxel Z-value and t-values in the CONN Gui for a given group contrast?

Thanks,

Jennifer

RE: Average within / between network connectivity

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[color=#000000]Dear Alfonso,[/color]

[color=#000000]thank you, this is very helpful information and a welcome suggestion for the first question so that I can present my results a bit more reviewer-friendly. I enjoy the use of CONN and am grateful for the quick feedback on the forum.[/color]

[color=#000000]Helene[/color]
[i]Originally posted by Alfonso Nieto-Castanon:[/i][quote][color=#000000]Dear Helene,[/color]

[color=#000000]Regarding (1) yes, you are exactly right that a Bonferroni correction of 6 (3 within-/between- network comparisons and 2-tailed) would suffice for these analyses (that is the number of multiple tests that are being evaluated by that script). If you prefer, you could also (perhaps a bit more standard) first convert the three uncorrected p-values output by the script into two-sided p-values (using a [b]p2 = 2*min(p,1-p)[/b] formula), and then apply FDR across those multiple tests (using a [b]P = conn_fdr(p2)[/b] command). That should be similarly valid and a bit less conservative than Bonferroni. [/color]

[color=#000000]Regarding (2), the former computation is used (i.e. the script averages the ROI-to-ROI connectivity values between all ROI pairs with ROI1 in set1 and ROI2 in set2)[/color]

[color=#000000]Hope this helps, and my apologies that this conn_withinbetween* script/functionality is still undocumented, I will eventually get around to making this part of the standard set of ROI/network analyses in CONN[/color]

[color=#000000]Alfonso[/color]
[i]Originally posted by Helene Veenstra:[/i][quote]Dear Alfonso,

Reviving an older thread as I am using this code line for my analyses, as I was interested in network connectivity changes related to covariates and groups. I have a couple of questions. 

1) since this analysis uses (uncorrected) two-tailed p-values; however draws from a small pool of regions to test, would it be appropriate to consider the results being adequately corrected with a Bonferroni correction for number of tests, and appropriate choice of number of tails?

Example: I investigate several hypotheses (different covariates/group comparisons) for the connectivity within two networks (SN, DMN) and the connectivity between those two. To obtain a p-value <0.05 per hypothesis, I use a corrected p-value with a Bonferroni correction of 6 (p<0.0083) based on 3 network (SN, DMN, SN-DMN) tests * 2 for two-tailed. 

2) as you explained this gives an average over all ROI connections within each chosen network. But how exactly is the between-network value calculated? As an average of every possible ROI (group1) to ROI (group2) connection? Or a calculated connectivity of the averaged connectivity over all ROIs within each group?[/quote][/quote]

Large cluster in 2nd level

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Hi,

I am running seed-to-voxel analysis (selecting seeds from network) and I am finding large clusters that are size of 50000 - 60000.
This seems to cover a lot of regions and hard to specify.
eg. cluster size 50000 covering frontal pole, precuneus, cingulate, lateral occipital gyrus, temporal pole, temporal gyrus...etc

Could you please help what is the best way to interpret this finding?

Thanks,


Jihyun

How to extract the first-level fisher-transformed values?

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Hi

I would like to extract the fisher transformed correlation values from BETA_Subject*_Condition*_Source*.nii. 

What would the next steps be?

Thanks for considering my request, any help would be greatly appreciated.


Yours truly
Larry

The file:[quote]conn_*/results/firstlevel/ANALYSIS_01/BETA_Subject*_Condition*_Source*.nii
contain the connectivity maps for each subject/condition/source (e.g. fisher transformed correlation values if using 'bivariate correlation' connectivity measures).[/quote]

Error using pinv

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Dear all,
I got this error during the preprocessing
ERROR DESCRIPTION:
Error using pinv
Too many input arguments.
Error in conn (line 1606)
dispdata{end+1}=fliplr(flipud(reshape(spm_get_data(files,pinv(files.mat)*txyz),dim(1:2))'));
CONN v.17.f
SPM12 + Anatomy DEM FieldMap MEEGtools
Matlab v.2016b
storage: 915.5Gb available
Does anyone know how to fix it?
Thanks
Luciano

Brain maps

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Hello, 

Would CONN work with rabbit brain images? Thank you.

- Conor

Is using ART for motion correction necessary anymore in Conn v17?

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Hi,
Do i still have to use ART toolbox with CONN v 17 or was there some motion artifact function that was added that i can use without resorting to ART?

Thank you

ICC and Global Correlation Differences

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Dear Alfonso and forum members,

I am currently working on a voxel-to-voxel connectivity analysis to assess potential correlations between connectivity values and a symptom scale.

I decided to compare the intrinsic connectivity and global correlation methods. According to the CONN manual, they are both measures of network centrality at each voxel and the former output is the root mean square of the correlation coefficient values while the latter provides the average of the correlation coefficient values. Therefore, I was not expecting my results to vary widely depending on the approach. However, they are completely different regardless of the contrast explored.

For example, a specific result of the contrast AllSubjects(0), SymptomScale(-1), Age(0), Sex(0) that survived the FWE correction using the ICC approach and had a cluster size of 300 voxels does not even appear when using the Global Correlation, at a significance level of p<0.001 uncorrected.

Can someone help me elucidate the reasons why my results are so different when using ICC or Global Correlation?

Thanks kindly, and I am sorry if this is mentioned somewhere obvious.

Best regards,
Maria

add new atlas

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Dear experts,

I would like to use my own atlas in my processing. Unfortunately I did not get the results properly.
I added the following statements in my batch script both for Setup and Analysis part:

 for nrois = 1
    batch.Setup.rois.names{nrois} = {'roi1.nii'};
    batch.Setup.rois.files{nrois} =fullfile(roipath,'roi1.nii');
    batch.Setup.rois.dimension{nrois} = {1};
 end

 batch.Analysis.sources.names = {'roi1.nii'};
 batch.Analysis.sources.dimension = {1};
 batch.Analysis.sources.driv = {0};

Do i need to change or add any statements in order to use my own roi atlas file?
Many thanks for considering my questions.

best regards,
Boshra

conn crashed after lack of storage

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Dear Alfonso and conn users,

I was running some analysis on conn and the system crashed during the night because of a lack of storage space. My storage space has then been increased but I can not load the project I was working on and I have the following error message :
Error using conn (line 726)
Failed to load file /home/k1467212/Documents/vortioxetine/merged_cns_oxford/conn_merged_cns_oxford_vortioxetine.mat.

Error in conn (line 4058)
conn('load',filename,true);
Error in conn_menumanager (line 120)
feval(CONN_MM.MENU{n0}.callback{n1}{1},CONN_MM.MENU{n0}.callback{n1}{2:end});
CONN v.17.f
SPM12 + DEM FieldMap MEEGtools
Matlab v.2015b
spm @ /home/k1467212/Documents/MATLAB/spm12
conn @ /home/k1467212/Documents/MATLAB/conn

Can someone help me to fix this error ?

Many thanks,

Ali

Preprocessed, Denoised rsfMRI images in Conn

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Hi,
I'm fairly new to the CONN toolbox, so apologise in advance for this very basic question.
I used CONN to preprocess and denoise my resting-state fMRI data set. 
Now I would like to use these preprocessed images for further analyses in SPM.

I just wanted to double check that the images I am after are the one with the prefix "swu". Are these the denoised images? Or are these the images until the smoothing preprocessing step?

Thanks very much in advance for your help,
Kati

RE: Slice order information in conn

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Dear Alfonso,

a follow-up question: is 15.h recent enough for the log? I doesn`t seem to work there. Preprocessing was done with that version, ,ay it be that tha slice order is saved somewhere else still?

Thank you,
Lucas
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