RE: Can the seed be reversed in functional connectivity analysis?

[color=#000000]Dear Pradeep,[/color]

[color=#000000]That's an interesting question. If you are performing the same second-level analyses using as seed the a target cluster found in a previous analysis, I would expect that certain amount of bias will be present in those secondary analyses (minimally on areas closely related to he original seed), so my impression is that it is fine to do those analyses as post hoc explorations to help you better interpret the context of the original analyses, but you should exercise care when considering effect-size estimates and cluster-level statistics on those secondary analyses since both of those can be reasonably expected to present some biases. [/color]

[color=#000000]Hope this helps[/color]
[color=#000000]Alfonso[/color]
[i]Originally posted by pradeepd:[/i][quote]Dear CONN community,

In functional connectivity (FC) analysis, can we use the results of a FC as a seed and do further FC analysis?
i.e. Use region A as seed and get region B as the result of a FC then use region B as seed to see where else do we get FC to in addition to region A.

Is such a reversed seed analysis accepted as valid? Or is it considered double dipping?

Thanks,
Pradeep
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Auditory Cognition Group,
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Website: http://www.auditorycognition.org/pradeep
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